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donkey anti sheep igg  (R&D Systems)


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    Structured Review

    R&D Systems donkey anti sheep igg
    Donkey Anti Sheep Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+sheep+igg/Donkey+Anti-Sheep+IgG+Biotinylated+Antibody/pm41448359-90-17-29
    Average 93 stars, based on 25 article reviews
    donkey anti sheep igg - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunodepletion:

    Article Title: Fibroblast Activation Protein Cleaves and Inactivates Fibroblast Growth Factor 21
    Article Snippet: Serum Isolation and Immunodepletion 6-week-old male C57BL/6 mice (The Jackson Laboratory) or 6- 8-week-old male Fap -deficient mice and littermate controls were used to isolate serum by incubating blood for >30 min at room temperature, followed by centrifugation at 1000 rpm for 10 min. One Shot TM FBS was purchased from Life Technologies and heat-inactivated at 56 °C for a minimum of 30 min. Human serum was obtained from healthy donors. .. For immunodepletion, 0.5 ml of serum was mixed with 10 μg of either a biotinylated sheep anti-hFAP polyclonal antibody (R&D Systems, catalog no. BAF3716) or biotinylated sheep IgG (R&D Systems, catalog no. BAF020) for 2 h at room temperature on a rotator. .. Protein-antibody complexes were separated using 0.2 ml of streptavidin magnetic M-280 Dynabeads (Thermo Fisher Scientific) for 30 min for a total of three rounds.

    Article Title: Fibroblast Activation Protein Cleaves and Inactivates Fibroblast Growth Factor 21
    Article Snippet: Serum Isolation and Immunodepletion––6 week old male C57BL/6 mice (Jackson Laboratory) or 6~8 week old male Fap-deficient mice and littermate controls were used to isolate serum by incubating blood for >30 min at room temperature, followed by centrifugation at 1,000 rpm for 10 min. One ShotTM fetal bovine serum (FBS) was purchased from Life Technologies and heat-inactivated at 56 °C for a minimum of 30 min. Human serum was obtained from healthy donors. .. For immunodepletion, 0.5 ml serum was mixed with 10 μg of either a biotinylated sheep anti-hFAP polyclonal antibody (R&D Systems, BAF3716), or biotinylated sheep IgG (R&D Systems, BAF020) for 2 h at room temperature on a rotator. .. Protein-antibody complexes were separated using 0.2 ml of streptavidin magnetic M280 Dynabeads (Thermo Fisher Scientific) for 30 min for a total of three rounds.

    Recombinant:

    Article Title: Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample
    Article Snippet: .. As a capture antibody, we used a rabbit polyclonal antibody raised against recombinant ANGPTL3 (aa 17-223) (BioVendor, Czech Republic) and as a detection antibody, a biotinylated sheep IgG raised against human recombinant ANGPTL3 (aa 17-460) (RnD Systems, Minneapolis, MN). ..



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